Impact of the COVID-19 pandemic on the hospital attendance of patients with primary cervical cancer in Heilongjiang, China.

Information regarding the impact of the coronavirus disease 2019 (COVID-19) pandemic on cervical cancer in mainland China is lacking. We explored its impact on the hospital attendance of patients with primary cervical cancer. We included 1918 patients with primary cervical cancer who initially attended Harbin Medical University Cancer Hospital between January 23, 2019, and January 23, 2021. Attendance decreased by 31%, from 1135 in 2019 to 783 in 2020, mainly from January to June (𝜒2 = 73.362, P < .001). The percentage of patients detected by screening decreased from 12.1% in January-June 2019 to 5.8% in January-June 2020 (𝜒2 = 7.187, P = .007). Patients with stage I accounted for 28.4% in 2020 significantly lower than 36.6% in 2019 (𝜒2 = 14.085, P < .001), and patients with stage III accounted for 27.1% in 2020 significantly higher than 20.5% in 2019 (𝜒2 = 11.145, P < .001). Waiting time for treatment was extended from 8 days (median) in January-June and July-December 2019 to 16 days in January-June (𝜒2 = 74.674, P < .001) and 12 days in July-December 2020 (𝜒2 = 37.916, P < .001). Of the 179 patients who delayed treatment, 164 (91.6%) were for the reasons of the healthcare providers. Compared to 2019, the number of patients in Harbin or non-Harbin in Heilongjiang Province and outside the province decreased, and cross-regional medical treatment has been hindered. The COVID-19 pandemic has negatively impacted cervical cancer patient attendance at the initial phase. These results are solid evidence that a strategy and mechanism for the effective attendance of cervical cancer patients in response to public health emergencies is urgently needed.

Focus on Marine Animal Safety and Marine Bioresources in Response to the SARS-CoV-2 Crisis.

SARS-CoV-2 as a zoonotic virus has significantly affected daily life and social behavior since its outbreak in late 2019. The concerns over its transmission through different media directly or indirectly have evoked great attention about the survival of SARS-CoV-2 virions in the environment and its potential infection of other animals. To evaluate the risk of infection by SARS-CoV-2 and to counteract the COVID-19 disease, extensive studies have been performed to understand SARS-CoV-2 biogenesis and its pathogenesis. This review mainly focuses on the molecular architecture of SARS-CoV-2, its potential for infecting marine animals, and the prospect of drug discovery using marine natural products to combat SARS-CoV-2. The main purposes of this review are to piece together progress in SARS-CoV-2 functional genomic studies and antiviral drug development, and to raise our awareness of marine animal safety on exposure to SARS-CoV-2.

SARS-CoV-2 Variants, RBD Mutations, Binding Affinity, and Antibody Escape.

Since 2020, the receptor-binding domain (RBD) of the spike protein of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been constantly mutating, producing most of the notable missense mutations in the context of "variants of concern", probably in response to the vaccine-driven alteration of immune profiles of the human population. The Delta variant, in particular, has become the most prevalent variant of the epidemic, and it is spreading in countries with the highest vaccination rates, causing the world to face the risk of a new wave of the contagion. Understanding the physical mechanism responsible for the mutation-induced changes in the RBD's binding affinity, its transmissibility, and its capacity to escape vaccine-induced immunity is the "urgent challenge" in the development of preventive measures, vaccines, and therapeutic antibodies against the coronavirus disease 2019 (COVID-19) pandemic. In this study, entropy-enthalpy compensation and the Gibbs free energy change were used to analyze the impact of the RBD mutations on the binding affinity of SARS-CoV-2 variants with the receptor angiotensin converting enzyme 2 (ACE2) and existing antibodies. Through the analysis, we found that the existing mutations have already covered almost all possible detrimental mutations that could result in an increase of transmissibility, and that a possible mutation in amino-acid position 498 of the RBD can potentially enhance its binding affinity. A new calculation method for the binding energies of protein-protein complexes is proposed based on the entropy-enthalpy compensation rule. All known structures of RBD-antibody complexes and the RBD-ACE2 complex comply with the entropy-enthalpy compensation rule in providing the driving force behind the spontaneous protein-protein docking. The variant-induced risk of breakthrough infections in vaccinated people is attributed to the L452R mutation's reduction of the binding affinity of many antibodies. Mutations reversing the hydrophobic or hydrophilic performance of residues in the spike RBD potentially cause breakthrough infections of coronaviruses due to the changes in geometric complementarity in the entropy-enthalpy compensations between antibodies and the virus at the binding sites.

The implementation and effectiveness of intergenerational learning during the COVID-19 pandemic: Evidence from China.

During the COVID-19 pandemic, many grandparents in China have spent more time with their grandchildren than they used to. When their adult children returned to work after a period of lockdown, many grandparents extended their roles from taking care of household tasks and looking after their grandchildren's basic needs to supervising their online learning and providing academic support. It has been a precious opportunity for both the children and their grandparents to get to know each other better and to learn from each other. During this challenging period of home learning, a Chinese initiative called the "Shaping Students' Vacation Life Project" (SSVLP), which is led by the Shanghai Municipal Institute for Lifelong Education (SMILE) of East China Normal University (ECNU), conducted a two-month project that investigated intergenerational learning between grandparents and grandchildren (IL-GP&GC) across seven primary schools located in six areas of China. They explored topics such as pandemic prevention, health and fitness, traditional culture and information literacy. Following this, the co-authors of this article conducted an interpretive inquiry to explore how the participating primary schools implemented the IL-GP&GC project, and to understand its impact. Based on in-depth interviews with 11 teachers and 7 families (including 7 grandchildren aged 7-13, and their 7 grandparents aged 60-68), four main findings emerged: (1) both generations gained more health knowledge, life skills and values; (2) the older generation changed their learning perspective and behaviours; (3) the younger generation understood their grandparents more and cultivated the concept of lifelong learning; and (4) the relationships between grandparents and grandchildren became closer.

Mise en œuvre et efficacité de l'apprentissage intergénérationnel pendant la pandémie de COVID-19 : résultats d'une enquête menée en Chine ­ En Chine, pendant la pandémie de COVID-19, beaucoup de grands-parents ont passé plus de temps que d'ordinaire avec leurs petits-enfants. Quand leurs enfants ont repris le travail en présentiel après le confinement, en plus d'assumer les tâches ménagères et de prendre soin des besoins fondamentaux de leurs petits-enfants, nombre d'entre eux ont étendu leur rôle à la surveillance de l'apprentissage en ligne et au soutien scolaire. Tant pour eux que pour leurs petits-enfants, ça a été l'occasion de mieux se connaître et la source d'un apprentissage réciproque. Pendant cette période difficile de l'école à la maison, un projet chinois appelé Projet d'organisation de la vie des élèves pendant les vacances (Shaping Students' Vacation Life Project/SSVLP), dirigé par l'Institut municipal de Shanghai d'éducation tout au long de la vie (Shanghai Municipal Institute for Lifelong Education/SMILE) de l'école normale supérieure de Chine orientale (East China Normal University/ECNU), a étudié deux mois durant l'apprentissage intergénérationnel entre grands-parents et petits-enfants (grandparents and grandchildren/IL-GP&GC) dans sept écoles primaires de six régions de Chine. Il s'est penché sur des sujets comme la prévention de la pandémie, la santé et la condition physique, la culture traditionnelle et la maîtrise de l'information. À la suite de cela, les auteurs de cet article ont mené une enquête d'interprétation pour examiner comment les écoles primaires participantes avaient mis en œuvre le projet d'apprentissage intergénérationnel entre grands-parents et petits-enfants et en comprendre l'impact. Cette enquête qui s'appuyait sur des interviews approfondies menées avec onze enseignants et sept familles (dont sept petits-enfants âgés de 7 à 13 ans et sept grands-parents âgés de 60 à 68 ans), a permis de dégager quatre constats principaux : (1) pour les deux générations, une amélioration des connaissances en matière de santé, des compétences nécessaires dans la vie courante et des valeurs a été constatée; (2) les anciens ont modifié leurs points de vue et comportements en matière d'apprentissage; (3) les jeunes ont appris à mieux comprendre leurs grands-parents et à cultiver le concept d'apprentissage tout au long de la vie, et (4) les liens entre les grands-parents et leurs petits-enfants se sont resserrés.

Current methods and prospects of coronavirus detection.

SARS-COV-2 is a novel coronavirus discovered in Wuhan in December 30, 2019, and is a family of SARS-COV (severe acute respiratory syndrome coronavirus), that is, coronavirus family. After infection with SARS-COV-2, patients often experience fever, cough, gas prostration, dyspnea and other symptoms, which can lead to severe acute respiratory syndrome (SARS), kidney failure and even death. The SARS-COV-2 virus is particularly infectious and has led to a global infection crisis, with an explosion in the number of infections. Therefore, rapid and accurate detection of the virus plays a vital role. At present, many detection methods are limited in their wide application due to their defects such as high preparation cost, poor stability and complex operation process. Moreover, some methods need to be operated by professional medical staff, which can easily lead to infection. In order to overcome these problems, a Surface molecular imprinting technology (SM-MIT) is proposed for the first time to detect SARS-COV-2 virus. For this SM-MIT method, this review provides detailed detection principles and steps. In addition, this method not only has the advantages of low cost, high stability and good specificity, but also can detect whether it is infected at designated points. Therefore, we think SM-MIT may have great potential in the detection of SARS-COV-2 virus.

A Hydrophobic-Interaction-Based Mechanism Triggers Docking between the SARS-CoV-2 Spike and Angiotensin-Converting Enzyme 2.

A recent experimental study found that the binding affinity between the cellular receptor human angiotensin-converting enzyme 2 (ACE2) and receptor-binding domain (RBD) in the spike (S) protein of novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is more than tenfold higher than that of the original severe acute respiratory syndrome coronavirus (SARS-CoV). However, main chain structures of the SARS-CoV-2 RBD are almost the same with that of the SARS-CoV RBD. Understanding the physical mechanism responsible for the outstanding affinity between the SARS-CoV-2 S and ACE2 is an "urgent challenge" for developing blockers, vaccines, and therapeutic antibodies against the coronavirus disease 2019 (COVID-19) pandemic. Taking into account the mechanisms of hydrophobic interaction, hydration shell, surface tension, and the shielding effect of water molecules, this study reveals a hydrophobic-interaction-based mechanism by means of which SARS-CoV-2 S and ACE2 bind together in an aqueous environment. The hydrophobic interaction between the SARS-CoV-2 S and ACE2 protein is found to be significantly greater than that between SARS-CoV S and ACE2. At the docking site, the hydrophobic portions of the hydrophilic side chains of SARS-CoV-2 S are found to be involved in the hydrophobic interaction between SARS-CoV-2 S and ACE2.